Figure 7 | Scientific Reports

Figure 7

From: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

Figure 7

Knocking down iNOS diminishes the cytoprotective effect of overexpressing CYGB. (a) Representative FACS analysis with annexin V/PI staining showing H2O2-induced apoptosis in hCPCs stably expressing scrambled or iNOS shRNA following with or without CYGB overexpression. (b) Quantitative data analysis for panel a. (c) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16. (d) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p < 0.05 vs. control; **indicates p < 0.01 vs. control; n = 3 independent experiments.

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