Figure 2 | Scientific Reports

Figure 2

From: Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

Figure 2

Assay of excision of the zeocin-resistance marker. Three different ZeoR transformants were shifted to YPM for induction and then streaking on YPD plate. The isolated single colony from YPD plate was spotted on both YPD and YPDZ plates. (1) Wild type P. pastoris GS115; (2) the transformant without methanol induction; 3–22: 20 isolated single colonies from YPD plate after methanol induction. The experiments were repeated three times.

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