Figure 3
From: Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris
Expression of Phy, Xyn and Arl in P. pastoris using self-excising vectors. Time dependence of phytase (a), xylanase (b) or lipase (c) activity and cell growth after induction with methanol. Black lines indicate phytase (a), xylanase (b) or lipase (c) activity, and gray lines indicate OD600. (a) Rhombuses indicate GS115/C-Phy, and circles indicate the control GS115/αE10; (b) rhombuses indicate GS115/C-Xyn, and circles indicate the control GS115/pPICHKA-xynA; (c) rhombuses indicate GS115/C-Arl, and circles indicate the control GS115/pPICHKA-epARL.
