Figure 6

Expression and release of the EGFR ligands AR, TGF-α and HB-EGF in response to WSPM2.5. NCI-H292 cells were exposed to 8 μg/ml WSPM2.5 for 2–36 h, after which RNA was extracted to determine AR, TGF-α and HB-EGF mRNA expression (A), or cell culture medium was collected to assess protein expression with ELISA (B). The data are expressed as the means ± SD; n = 3. *P < 0.05 compared with the control.