Figure 4
From: TREM2 promotes Aβ phagocytosis by upregulating C/EBPα-dependent CD36 expression in microglia
Deficiency of TREM2 signaling impairs the phagocytic activity of microglia. (A) Primary microglia of WT or KO mice were stained with PE-conjugated anti-CD11b or isotype control antibody, and the purity of primary microglia was determined using flow cytometric analysis. (B) Primary microglia were treated with 8 μM Aβ in the presence of 10 μM cytochalasin D (CD) as an inhibitor of actin-mediated phagocytosis, and the percentage of Aβ phagocytosed was determined using the phagocytosis assay. (C) The percentage of Aβ phagocytosed by BV2 cells after treatment with anti-TREM2 (1 μg/ml). (D) The percentage of Aβ phagocytosed by BV2 cells after treatment of soluble TREM2 (sTREM2, 2 μg/ml). (E) Representative fluorescence microscopic views (20×) from brain slices of 5XFAD mice stained with Thioflavin T after co-culture with primary microglia. Scale bar: 200 μm (F) The numbers of Aβ plaques quantified from the brain slice assay in (E). Data shown are representative of three independent experiments, and the error bars indicate the mean ± SEM of three independent experiments. (*p < 0.05; **p < 0.01).
