Figure 5
From: TREM2 promotes Aβ phagocytosis by upregulating C/EBPα-dependent CD36 expression in microglia
TREM2 promotes Aβ phagocytosis by upregulating CD36 in microglia. (A,B) CD36 mRNA expression was determined by semi-quantitative RT-PCR from primary microglia (left), and the relative mRNA expression levels were quantitatively analyzed by normalization against GAPDH (right panels) (full-length blots/gels are presented in Supplementary Fig. S8). (C) Immunofluorescent staining of primary microglia. (D) Surface expression of CD36 on primary microglia analyzed using flow cytometry. (E) BV2 cells were treated with recombinant CD36 (rCD36, 1 μg/ml) in the presence of Aβ (8 μM) at 24 h after transfection with a control vector or a plasmid encoding TREM2, and the phagocytosis assay was performed. (F) The percentage of phagocytosed Aβ in primary microglia infected with lentivirus encoding shRNA targeting CD36. Data shown are representative of three independent experiments, and the error bars indicate the mean ± SEM of three independent experiments. (*p < 0.05; **p < 0.01).
