Figure 7
From: Quantification of Aquaporin-Z reconstituted into vesicles for biomimetic membrane fabrication
Gel electrophoresis results of AqpZ incorporated vesicles. (a–c) Electrophoretic analysis of E. coli lipid samples before and after incorporation via dialysis, full length gels are presented in Supplementary Figure S2(a–c). Samples were derived from the same experiment and the gels were processed using same settings in parallel. Sample containing 1 ug of AqpZ or equivalent volume of control buffer is loaded for gel electrophoresis. Duplicates of each sample were loaded for each gel electrophoresis for (a–b). Standard deviation is calculated from results obtained from three independent repeated experiments. Before and after refer to before and after dialysis respectively. Pellet, wash, and supernatant refers to the different fractions collected from ultracentrifugation separation process. ECC and ECZ refer to control and AqpZ reconstituted E. coli lipid vesicles respectively. ECCNi and ECZNi refer to nickel labeled control and AqpZ reconstituted E. coli lipid vesicles respectively. NiZ refers to nickel labeled AqpZ and Z refers to non-nickel labeled AqpZ. (d) BSA standard working curve and quantified AqpZ results.
