Figure 2 | Scientific Reports

Figure 2

From: Selective antibody activation through protease-activated pro-antibodies that mask binding sites with inhibitory domains

Figure 2

Removal of inhibitory domains from recombinant pro-antibodies by MMP-2. Unmodified anti-EGFR antibody (A), CBa-anti-EGFR antibody (B), C2b-anti-EGFR antibody (C) or LAP-anti-EGFR antibody (D) were digested by MMP-2 for the indicated time. Digested antibodies were resolved by reducing SDS-PAGE and analyzed by western blot using HRP-conjugated goat anti human-IgG Fcγ antibodies. Note that amounts of cut (mature form) antibodies increased as digestion was prolonged from 1 minute to 60 minutes. ID-HC: inhibitory ___domain-heavy chain. HC: heavy chain. with MMP: with MMP-2 incubation. The original blots image for panel A–D are presented in Supplementary Figure S5.

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