Figure-1

Effects of BEV and/or RES on VEGF and its receptors. Bar graph shows the relative gene expressions of vegf and its receptors r1 and r2 with respect to mRNA levels of gapdh in ARPE-19 cells treated with BEV, RES and BEV + RES (A). Representative immunofluorescent images showing the VEGF (green) and DAPI (blue) staining in ARPE-19 cells after various treatments (B). Mean fluorescent intensity measured using Image J software and represented graphically (C). Graphical representation of the secreted VEGF concentration was measured using sandwich ELISA (D). Representative western blot analysis for VEGF, VEGF-R2 and GAPDH in ARPE-19 cells incubated with BEV, RES, BEV + RES and untreated cultures (E). Graphical representation of the quantified western blot analysis with respect to GAPDH protein expression (F). Statistical analysis was performed using student’s t-test (**P < 0.01, P*** < 0.005) (n ≥ 3). Scale bar = 5 µm.