Figure 9
Cortical optogenetic stimulation normalizes the increased mIPSC amplitude of layer V pyramidal neurons in tSCI mice. (A) Representative mIPSC traces of the 3 groups at a holding potential of + 10 mV. (B) There were no significant differences in the mean frequency of mIPSCs among all groups (sham: 11.5 ± 0.7 Hz; tSCI: 13.0 ± 0.5 Hz; tSCI + LED: 11.8 ± 0.9 Hz. p > 0.05). (C). The amplitude of mIPSC in tSCI group was significantly higher than the sham group (Sham: 13.3 ± 0.2 pA; tSCI: 14.0 ± 0.2 pA. p < 0.01), which became decreased in the tSCI + LED group (tSCI + LED: −13.1 ± 0.2 pA. p < 0.01 when the tSCI and tSCI + LED groups were compared). (D-E). There were no significant differences in cumulative distributions of mIPSC frequencies and amplitudes (p > 0.05, K-S test).
