Figure 4
P2rx7 −/− mice have increased body weight and abnormal glucose, insulin, triglyceride and cholesterol levels which could be associated to apparent resistance to insulin. (A) The weight of six P2rx7 +/+ and eight P2rx7 −/− male mice was measured every week between 9–10 h AM starting at weaning (3-week-old mice) to 20-week-old mice. The results are expressed as the mean ± SEM and statistical significance was determined by an unpaired t test, where *p < 0.05. (B) The Area Under the Curve (AUC) of the weight of experimental animals was determined by the trapezoidal method and expressed as the mean ± SEM. The statistical significance was determined by an unpaired t test, where **p < 0.01. Serum biochemical analyses were realized on 12-week-old male mice (9 P2rx7 +/+ and 9 P2rx7 −/−) following 6h of diurnal fasting. Glucose (C), triglyceride (TG) (D) and total cholesterol (E) concentrations were determined using an automated clinical analyzer as described in the materials and methods section, whereas serum insulin concentration (F) was measured by an ELISA test. Results are presented as the mean ± SEM, where *p < 0.05 as determined by unpaired t tests. The HOMA2-IR (G) and HOMA2-%B (H) indices were calculated from the glucose and insulin levels found in panels C and F using the HOMA calculator presented in the M&M section. Results are expressed as the mean ± SEM. Statistical significance was determined by an unpaired t test, where *p < 0.05.
