Figure 7
The expression of Acaca, Fasn and Acox1 is downregulated in the liver of P2rx7 −/− mice. (A) Livers of control wild-type littermates (open circles) and of P2rx7 −/− mice (close circles) were harvested and mRNA extracted prior to quantitative real-time PCR assay. The relative mRNA expression was expressed as the ratio of the expression over TATA-box binding protein gene expression (Tbp). Results are presented as the mean ± SEM, where *p < 0.05 as determine by multiple t tests. Liver lysates were also obtained and (B) ACC and (C) FAS protein expression determined by Western blots. β-Tubulin expression was used as a control of protein integrity and to ensure equal loading. Signal intensity was quantified by densitometry and reported as the mean ± SEM of the ratio of protein expression over β-tubulin, where *p < 0.05 as determine by an unpaired t tests. (D) Hepatic levels of AMPK phosphorylation of Thr172 and protein expression for AMPK, PPARα, and PPARβ determined by Western blots. GAPDH expression was used as a control of protein integrity and to ensure equal loading.
