Figure 1 | Scientific Reports

Figure 1

From: The thermogenic actions of natriuretic peptide in brown adipocytes: The direct measurement of the intracellular temperature using a fluorescent thermoprobe

Figure 1

The experimental protocol showing the duration and the time course of the intracellular temperature measurement. Brown and white adipocytes treated with the cellular thermoprobe were incubated at 37 °C, and were placed in the microscopic chamber at 35 °C or 37 °C. The fluorescence intensity and ratio were measured every 6 minutes. After stabilization for 40 minutes, atrial natriuretic peptide (ANP), isoproterenol, or CL316,243 was added to the indicated sample dish.

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