Figure 5
From: Chemodrug delivery using integrin-targeted PLGA-Chitosan nanoparticle for lung cancer therapy
Cell cycle and apoptosis analysis in nanoparticle-treated lung tumor cells. (A) Graphical representation of percentage population of cells in G1, S and G2/M phases of cell cycle at 24 h and 48 h following treatment with treated with either 12.5 nM (A549) or 28.0 nM (H1299) equivalents of PTX in free, PTX-PLGA-CSNP or PTX-PLGA-CSNP-RGD formulations. (B) Dot plot (left) and graphical representations (right) of total apoptotic cells obtained by flow cytometry analysis of Annexin V- Alexa Fluor 488/PI stained A549 and H1299 cells at 24 h and 48 h time points. Cells were treated with either 12.5 nM (A549) or 28.0 nM (H1299) equivalents of PTX in free, PTX-PLGA-CSNP or PTX-PLGA-CSNP-RGD formulations. Total apoptotic cells (%) were the sum of quadrants Q2 and Q3. (n = 3); *p < 0.05, **p < 0.01; Statistical analysis was represented for G2 populations between treatment groups. (n = 3); *p < 0.05; **p < 0.01; NS, non-significant.
