Figure 3 | Scientific Reports

Figure 3

From: Identification of host DEAD-box RNA helicases that regulate cellular tropism of oncolytic Myxoma virus in human cancer cells

Figure 3

Effects of selected RNA helicase siRNAs on replication of MYXV in non-permissive PANC-1 cells. PANC-1 cells were seeded, transfected with siRNAs, and infected with vMyx-GFP-TdTomato virus as described in Fig. 2. The cells were harvested at 72 hpi to determine progeny virus formation by titration assay on permissive RK13 cells. (A) Overlay showing the cells expressing both GFP and Tdtomato (yellow color); (B) The virus titers were determined in triplicate following serial dilution onto RK13 cells. Statistically significant differences in comparison to infection with MYXV alone are indicated; *p < 0.05; **p < 0.01; ***p < 0.001.

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