Figure 4
Effects of selected RNA helicase siRNAs on replication of MYXV in permissive human A549 cells. A549 cells were plated in 48 well plates, transfected with control siRNA or siRNAs targeted to the indicated RNA helicases. After 48 h the cells were infected with vMyx-GFP-TdTomato with the indicated MOI and virus-containing media was replaced with fresh media after 1 h of virus adsorption. The cells were harvested at the indicated time points to determine progeny virus formation by titration assay on permissive RK13 cells. Images of the siRNA-transfected and virus- infected A549 cells: (A) infected with MYXV at MOI of 0.01 FFU/cell and images were taken 3 days post infection (p.i.) (upper panels) or 6 days p.i. (lower panels); (B) foci size from images taken on 3 days post infection were measured using image J software and plotted. At least 8–10 foci were measured for each siRNA treatment. Statistically significant differences compared to infection with MYXV alone are indicated; **p < 0.01. (C) The virus titers after 48 and 96 hrs post infection with MOI of 0.01 are shown. (D) Table showing fold changes in virus titer after transfection of selected RNA helicase siRNAs and infection with different MOIs compare to the infection with MYXV alone. *p < 0.05; **p < 0.01; n.s., not significant.
