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Figure 1

From: Preparation and functional evaluation of collagen oligopeptide-rich hydrolysate from fish skin with the serine collagenolytic protease from Pseudoalteromonas sp. SM9913

Figure 1

Effects of key factors on extracellular protease production of SM9913. (a) Effect of inoculation amount in shake flask fermentation. (b) Effect of gas flow in a 3 L fermentor. (c) Effect of stirring speed in a 3 L fermentor. Line 1, fermentation with a stirring speed of 400 rpm in the first 48 h and 350 rpm in the remaining time; line 2, fermentation with a stirring speed of 500 rpm in the first 48 h and 450 rpm in the remaining time; line 3, fermentation with a stirring speed of 500 rpm in the whole process. (d) Protease activity in the broth of SM9913 fermentation in a 200 L fermentor. Line 1, fermentation with a gas flow of 2 m3/h and a stirring speed of 90 rpm; line 2, fermentation with a gas flow of 4 m3/h and a stirring speed of 80 rpm; line 3, fermentation with a gas flow of 6 m3/h and a stirring speed of 100 rpm.

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