Figure 2
From: A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris
Testing the efficiency of linearised plasmidis DNA versus PCR products using pPICZαA. DNA was prepared by PmeI digest or PCR, where the latter was processed with reaction clean-up (C&C) or gel extraction (GE). For each transformation, 198.6 ng DNA was transformed into electrocompetent GS115 cells from the same batch. Colonies were counted and the results expressed as a fold change in colony count, compared to the PmeI digest. Error bars indicate the standard deviation in four biological repeats, where both separate DNA and batches of electrocompetent cells were prepared.
