Figure 4

Phylogeny of CM-sec (a) and CM-nonsec (b). Both analysis utilized the first 250 comparison hits from Blastp, standardizing bars to 0.4 residues. It was noticed that for CM-sec the majority of analyzed organisms are plant-associated organisms (check for details at Fig. S10 and S11 – Supporting Information) which are found within three different classes (α, β and γ-proteobacterias), while for CM-nonsec all the analyzed microorganisms are introduced in γ-proteobacteria class maintaining the structural pattern of order belonging to this class. In addition, it is possible to observe that frequency of non-synonymous mutations is smaller in CM-sec indicating that this copy was more conserved during the evolution of the species.