Table 1 Variation in TYLCV populations obtained from the TYLCV-infected tomato and viruliferous B. tabaci MED and B. tabaci Asia II 1.
From: Molecular variation of tomato yellow leaf curl virus in the insect vector Bemisia tabaci
Host | Days p.i. | Replicate | % of mutant clone | Total mutations/bases sequenced | Mutation frequencyb |
|---|---|---|---|---|---|
tomato | 30 | 1 | 29.4% (5/17) | 5/47277 | 1.06 × 10−4 |
2 | 40% (8/20) | 9/55620 | 1.62 × 10−4 | ||
3 | 52.9% (9/17) | 16/47277 | 3.38 × 10−4 | ||
Total | 40.77% | 30/150174 | 2.02 × 10 −4 a | ||
45 | 1 | 58.8% (10/17) | 11/47277 | 2.32 × 10−4 | |
2 | 50% (9/18) | 14/50058 | 2.80 × 10−4 | ||
3 | 61.1% (11/18) | 12/50058 | 2.39 × 10−4 | ||
Total | 56.60% | 37/147393 | 2.5 × 10 −4 a | ||
90 | 1 | 70.6% (12/17) | 21/47277 | 4.44 × 10−4 | |
2 | 64.7% (11/17) | 13/47277 | 2.75 × 10−4 | ||
3 | 66.7% (12/18) | 19/50058 | 3.8 × 10−4 | ||
Total | 67.30% | 53/144612 | 3.66 × 10 −4 a | ||
MED | 0.5a | 1 | 18.75% (3/16) | 3/44496 | 6.74 × 10−5 |
2 | 45% (9/20) | 18/55620 | 3.23 × 10−4 | ||
3 | 35% (7/20) | 8/55620 | 1.43 × 10−4 | ||
Total | 32.92% | 29/155736 | 1.78 × 10 −4 a | ||
15 | 1 | 93.3% (14/15) | 139/41715 | 3.33 × 10−3 | |
2 | 94.4% (17/18) | 156/50058 | 3.12 × 10−3 | ||
3 | 100% (15/15) | 199/41715 | 4.77 × 10−3 | ||
Total | 95.9% | 494/133488 | 3.74 × 10 −3 b | ||
Asia II 1 | 0.5a | 1 | 33.3% (4/12) | 15/33372 | 4.49 × 10−4 |
2 | 7.69% (1/13) | 1/36153 | 2.76 × 10−5 | ||
3 | 4.54% (1/22) | 1/61182 | 1.63 × 10−5 | ||
Total | 15.18% | 17/130707 | 1.643 × 10 −4 a | ||
15 | 1 | 100% (12/12) | 117/33372 | 3.50 × 10−3 | |
2 | 100% (11/11) | 104/30591 | 3.40 × 10−3 | ||
3 | 100% (12/12) | 114/33372 | 3.42 × 10−3 | ||
Total | 100% | 335/97335 | 3.44 × 10 −3 b | ||
Rolling circle amplification control |  | 5%(1/20) | 1/55620 | 1.80 × 10 −5c | |
