Figure 3 | Scientific Reports

Figure 3

From: ATF4 overexpression induces early onset of hyperlipidaemia and hepatic steatosis and enhances adipogenesis in zebrafish

Figure 3

Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of selected lipogenic and UPR target genes in ATs. The relative mRNA expression of lipid transport (A), lipogenic factor (B), lipogenic enzyme (C), and UPR target (D) genes in ATs − Dox compared with gene expression in controls (ATs + Dox and WT ± Dox). The qRT-PCRs were performed in triplicate. Expression analysis of the selected genes using cDNA prepared from average 2–3 four months male–female fish pairs per groups (WT-Dox, WT + Dox, AT1-Dox, AT1 + Dox, AT2-Dox, and AT2 + Dox). Levels of mRNA were normalized to β-actin and expressed as fold of values in the WT-Dox control. Values were normalized against β-actin. The asterisk represents statistically significant differences; *p < 0.01, and **p < 0.005.

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