Figure 4

Effects of CIK combined with ATRA on colony formation and cell apoptosis of A549 and NCI-H520 cells. (A) Cell colony formation was significantly inhibited by treatment with CIK combined with ATRA. (B) CIK combined with ATRA treatment effectly increased the total apoptosis of A549 and NCI-H520 cells detected by annexin V-phycoerythrin/7-amino-actinomycin D flow cytometry assay. CIK or ATRA alone treatment also increased the apoptosis to a certain extent at the early or late stage. The number of colonies was calculated and plotted on the histogram (n = 3). *p < 0.05,**p < 0.01 compared with control groups, respectively. Δp < 0.05 compared with CIK or ATRA group, respectively.