Figure 3 | Scientific Reports

Figure 3

From: KIF1Bβ increases ROS to mediate apoptosis and reinforces its protein expression through O 2 − in a positive feedback mechanism in neuroblastoma

Figure 3

KIF1Bβ requires ROS, specifically O2 −, to induce apoptosis in neuroblastoma cells. (A) Immunoblot analysis of differentiated PC12 cells with (+) and without (−) NGF as indicated. Differentiated PC12 cells were treated with or without (control) NAC prior to NGF withdrawal and replenished every 12 hours after NGF withdrawal for treated samples. (B) Immunoblot analysis of CHP212 cells transfected with 4 µg FLAG-KIF1Bβ600–1400 for 24 hours followed by increasing doses of DPI treatment for 12 hours. Bottom – corresponding densitometry for cleaved caspase-3 (CC3) expression (mean ± SD; n = 3; **P < 0.01; ****P < 0.0001). (C) Immunoblot analysis of NB1 cells transfected with 4 µg FLAG-KIF1Bβ600–1400 for 24 hours followed by increasing doses of Tiron treatment for 6 hours.

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