Figure 5 | Scientific Reports

Figure 5

From: Insulin-like peptide 3 expressed in the silkworm possesses intrinsic disulfide bonds and full biological activity

Figure 5

ETD-MS/MS analysis of the disulfide-linked peptides. (A) MS/MS spectra of the non-reduced ion with an m/z value of 648.6 (3+) from peak I in total ion chromatogram displayed characteristic peptide fragments P1 (L13CGHHFVR20) and P2 (H94CCLSGCTR102), indicating that Cys14 in P1 forms a disulfide bond with one of the cysteines in P2. (B) MS/MS spectra of the non-reduced ion with an m/z value of 546.9 (3+) from peak II in total ion chromatogram also generated distinctive peptide fragments P3 (L25CGGPR30) and P4 (Q103DLLTLCPH111), indicating that Cys26 in P3 forms an intermolecular disulfide bond with Cys109 in P4.

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