Figure 3
Local inflammation in cardiac left ventricular tissue 72 h after multiple trauma or sham procedure. (A) Elevation of proinflammatory cardiodepressive cytokine IL-1β concentration in left ventricular homogenates after multiple trauma in both treatment groups DCO (grey bar) and ETC (black bar) compared to animals after sham procedure (white bar) assessed by ELISA. (B) Elevation of proinflammatory cardiodepressive cytokine IL-6 concentration in left ventricular homogenates after multiple trauma in ETC (black bar) treatment group compared to animals after sham procedure (white bar) assessed by ELISA. (C) Representative western blot of C5aR1 in left ventricular homogenates in pigs after trauma and DCO (grey bar) or ETC (black bar) treatment and in sham treated animals (white bar), pixel densitry was assessed for quantification. Representitive C5aR1 staining of left ventricle tissue after trauma and DCO (middle) or ETC (right) treatment and in sham (left) treated animals (D). (E) Changes in density of C5aR1 staining in left ventricular tissue after trauma and DCO (grey bar) or ETC (black bar) treatment and in sham treated animals (white bar). (F) Changes in C5aR2 protein expression in left ventricular tissue after trauma and DCO (grey bar) or ETC (black bar) treatment and in sham treated animals (white bar). For each bar n = 6 samples, *p < 0.05.
