Figure 5
Representative confocal imaging of α2-actinin (A) and desmin (C) in pig left ventricle tissue sections. Blue staining of nuclei with DAPI, α2-actinin labeled with Alexa Flour® 488 (green), desmin labeled with PE (red). α2-actinin staining in left ventricles of sham treated animals (left) or 72 h after multiple trauma and DCO (middle) or ETC (right) treatment. Flourescence intensity of α2-actinin staining and confocal imaging in sham (white bar), multiple trauma and ETC treatment (grey bar) or ETC treatment (black bar) (B). Desmin staining in left ventricles of sham (left) treated animals or 72 h after multiple trauma and DCO (middle) or ETC treatment (right). Flourescence intensity of desmin staining and confocal imaging in sham (white bar), multiple trauma and ETC treatment (grey bar) or ETC treatment (black bar) (D). Scale bar represents 10μm. Representation of flourescence intensity in arbitrary units (AU) (D), n = 6, p < 0.05.
