Figure 2 | Scientific Reports

Figure 2

From: Development of a yeast internal-subunit eGFP labeling strategy and its application in subunit identification in eukaryotic group II chaperonin TRiC/CCT

Figure 2

Visualization of the inserted eGFP tag in reference-free 2D class averages. (A) For TRiC CCT1-eGFP in the both-ring closed conformation, we can visualize the exposed extra density corresponding to the inserted eGFP tag (indicated by yellow arrow) from the reference-free 2D class averages especially in the side views. (B,C) For TRiC CCT2-eGFP in the closed state and TRiC CCT5-eGFP in the open state, although shadow density exposed outside of TRiC can be observed in the reference-free 2D class averages, the feature is rather fuzzy and it is hard to identify which subunit the tag belongs to.

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