Figure 6
The potential mechanisms underlying the cell death induced by IONPs in SKOV-3 cells. (A) Apoptotic and necrotic cell death were analyzed by Annexin V and PI dual staining after IONPs treatment for 24 h in SKOV-3 cells. Intracellular reactive oxygen species (ROS) production in SKOV-3 cells treated with various IONPs for 18 h was detected using a H2DCFDA probe by confocal microscopy (B) and flow cytometry (C), respectively. (D) The measurement of membrane mitochondria potential (MMP) of SKOV-3 cells treated with different concentrations of SEI-10 for 18 h. (E) the effect of IONPs on the cell cycle in SKOV-3 cells after 24-h treatment. (F) The effects of IONPs on the expression of proteins involved in apoptosis (Bcl-2, Bax), cell cycle (Cyclin D), and autophagy (LC3B-II), determined by western blot analysis after 18-h treatment.
