Figure 1
From: Differences in the mechanical unfolding pathways of apo- and copper-bound azurins
Mechanical unfolding of apo-azurin. (A) (On left) Schematic representation of SMFS experiment on azurin polyprotein. (On right) Structure of holo-azurin (PDB code: 4AZU). (B) A representative force-versus-extension (FX) trace of apo-azurin, pulled at 400 nm/s, showing parallel unfolding pathways. Zoomed-in panel shows the trace in more detail. Worm-like chain (WLC) fits to the force peaks of native state, Napo (blue) and intermediate, Iapo (green) are shown. More FX data is given in Fig. S8. (C) Histogram of the ΔLc of the unfolding of azurin from Napo to Uapo (blue) and from Napo to Iapo (green). (D) Histogram of unfolding force of Napo (blue) and Iapo (green). Errors are SD. (E) Scatterogram of rupture forces of Iapo vs Napo for FX traces showing intermediate. Error bars are SD.
