Figure 4
From: Differences in the mechanical unfolding pathways of apo- and copper-bound azurins
Mechanical unfolding of holo-azurin. (A) Representative FX trace of holo-azurin showing intermediate. Zoomed-in panel shows the trace in more detail. WLC fits are shown for the unravelling of native state, Nholo (blue) and intermediate, Iholo (green). More FX data is given in Fig. S10 in the Supplementary Material. (B) Unfolding events of holo-azurin that followed 4-state pathways: Nholo to Iholo to I’holo to Uholo. WLC fits are shown for native state, Nholo (blue), intermediate, Iholo (green), second intermediate, I’holo (red). (C) Histogram of the total change in contour length (ΔLc) of unfolding of azurin from Nholo to Uholo (blue), Nholo to Iholo (green), and Iholo to I’holo (red). Errors are SD. ΔLc histogram for Napo to Uapo (black) is also shown. (D) Histogram of unfolding force of azurin from rupture of Nholo (blue), Iholo (green), and I’holo (red). Errors are SD. (E) Scatterogram of Iholo peak force vs Nholo peak force for unfolding events showing intermediate (Iholo). Error bars are SD.
