Figure 3
From: Visualization and quantification of dynamic STAT3 homodimerization in living cells using homoFluoppi
Analysis of STAT3 inactivation using homoFluoppi. (a) Cells stably expressing PB1-mAG1-STAT3 were treated with 1 ng/mL OSM for 30 min. Then OSM was washed out and cells were incubated in medium without OSM for the indicated time. As a control experiment, cells without OSM stimulation and cells without OSM washing are shown. Each point represents the mean of four replicates, and the error bars represent the standard deviation from the mean. (b) Representative fluorescent images of cells fixed at the indicated condition. Images of mAG1 (green) and Hoechst33342-stained nuclei (blue) were merged. Scale bars, 50 μm. (c) Western blot analysis of cells obtained in the same condition as shown in (a). Full-length blots are presented in Supplementary Figures 5.
