Figure 4

HSP27 inhibitor decreased the expression of inflammation-related molecules. (A) cDNA was synthesized from the total RNAs extracted from mouse lungs exposed to X-rays and subjected to quantitative real-time PCR analysis for inflammation-related molecules. (B) Immunohistostaining of TGF-β (B) and IL-1b (C) using mice lung tissues. Quantification of stained tissue was presented as mean ± standard error. *P < 0.05, **P < 0.01 and ***P < 0.001 versus control; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. IR. Control, untreated; IR, 75 Gy irradiation (IR); IR + J2, irradiation + 15 mg/kg J2; IR + AMI, irradiation + 100 mg/kg amifostine; J2, 15 mg/kg J2 only and AMI, 100 mg/kg amifostine only.