Figure 1

Triple-label immunofluorescence localization of BeWo cytotrophoblasts (left) and syncytiotrophoblasts (right). BeWo cells were treated with vehicle control (left) or with forskolin (right), and after 72 hours, cells were fixed, stained with DAPI (blue), anti-ZO1 (red), or anti-hCGβ (green), and imaged with an Olympus confocal microscope.