Figure 2

Hippocampal damage in Theiler’s murine encephalomyelitis virus (TMEV)-infected SJL mice following IL-10R blockade resembles lesions in C57BL/6 mice at 7 days post infection. (a) Representative image of a hippocampus derived from a TMEV-infected, isotype-treated SJL mouse (control). (b) Severe inflammation accompanied by neuronal pyknosis in an infected SJL animal following IL-10R blockade. (a,b) H&E staining. (c) Semiquantitative scoring of hippocampal inflammation. (d) NeuN⁺ neurons in an intact pyramidal layer of a TMEV-infected isotype-treated SJL mouse (control). (e) Loss of NeuN⁺ neurons following IL-10R blockade in a TMEV-infected SJL animal. (d,e) NeuN-specific immunohistochemistry (IHC). (f) Morphometric analysis of NeuN-specific staining in the hippocampus. (g) TMEV-infected, isotype-treated SJL mouse without detectable axonal injury (control). (h) Accumulation of β-amyloid precursor protein (β-APP) in a swollen axon (arrow) after TMEV-infection and IL-10R blockade in a SJL mouse. (g,h) β-APP-specific IHC. (i) Quantification of β-APP⁺ axons in the hippocampus and corpus callosum. (c,f,i) Box plots display median and quartiles with minimum and maximum values. *Significant difference p ≤ 0.05 (Mann-Whitney U test).