Figure 5

LamB is required for growth in hMDMs. To determine intra-vacuolar replication of the WT strain, the dotA mutant, the lamB mutant, catalytic mutants (D193A, E227A, and D296A), and complemented lamB mutant (lamB/C), hMDMs were infected and number of CFUs were determined at 2, 8, and 24 h post-infection. Data points represent (mean CFUs ± SD, n = 3) and are representative of three independent experiments.