Figure 2
From: mTOR complexes differentially orchestrates eosinophil development in allergy
mTOR knockdown promotes IL-5-induced colony forming units. (a) 2 × 105 NAMNCs originated from Mtorflox/flox mice were seeded in methylcellulose-based semi-solid medium for colony formation in the presence of IL-5 (10 ng/ml). CFUs were calculated at day 7, and images were captured by microscope. (b) CFU numbers after adenoviral Cre recombinase expression. (c) CFUs were picked and washed by PBS, then stained with PE-conjugated SiglecF, representative histograms are shown. (d) Torin-1 (250 nM) was added in the medium before being seeded, and the number of CFUs were counted on day 7. All the results above were triplicated by three independent experiments and were analysed by Student’s T-test as means ± SEM. *P < 0.05, ***P < 0.001.
