Figure 6

Myeloid knockout of mTOR deteriorates OVA-induced allergic airway inflammation. (a) Two strains of mice received ovalbumin (OVA) mixed with an injection of alum adjuvant on day 0 and day 14 for sensitization, then challenged with 1.5% OVA by nebulization. 24 hours after the last OVA exposure, mice were anaesthetized and sacrificed. Bronchoalveolar lavage fluids (BALFs) were obtained and total cells and eosinophils in BALFs were counted. (b,c) Representative histological images are shown (b) Inflammation scores were calculated according to H&E slides. (d and e) Bone marrow cells were also harvested and red blood cell lysis was performed. Eosinophil percentage was detected (d) and statistically analysed (e). (f–i) Th2 cytokines were detected by lung homogenates. F, mRNA expression of IL-4. G, mRNA expression of IL-13. H, protein concentration of IL-4. I, protein concentration of IL-13. Results were analysed as means ± SEM. All the results above were repeated by three independent experiments (n = 8 per group). *P < 0.05, **P < 0.01, ***P < 0.001.