Figure 6
The treatment of proteolytic enzymes on MT-MEF. (A) Immunofluorescence analysis of fibronectin and collagen-IV expressions in MT-MEF and MMC-MEF. (B) Immunofluorescence analysis of fibronectin and collagen-IV expressions in MT-MEF (Ctrl) and MT-MEF treated by collagenase-IV. (C) J1 cells cultured on MT-MEF were treated by collagenase-IV for 5, 10, and 20 min. (D) Percentage of J1 cell adherent on MT-MEF treated by collagenase-IV for different times. (E) qRT-PCR analysis of expression of naïve and primed markers in J1 cells cultured on MT-MEF (Ctrl) and MT-MEF treated by collagenase-IV. (F) Diagram of interaction between different feeder cell niches and pluripotent stem cells. Ctrl, MT-MEF without treatment. Scale bar, 100 μm for A and B, 200 μm for C. Data indicate mean ± SD, *P < 0.05, **P < 0.01, n = 3.
