Figure 5 | Scientific Reports

Figure 5

From: Alcohol consumption promotes colorectal carcinoma metastasis via a CCL5-induced and AMPK-pathway-mediated activation of autophagy

Figure 5

CCL5 induces autophagy through the AMPK signaling pathway. (a,b) Different expression genes in autophagy path way in HT29 and DLD-1cells with stimulated by CCL5 through High-throughput sequencing. (c–f) HT29 and DLD-1cells with stimulated by CCL5 as described in methods and materials and then the expression of ULK1, CAMKK2 and phosphorylated AMPK tyrosine 172 were analyzed by western blot. The expression of β-Actin used as a loading control. (g–j) HT29 and DLD-1 cells were pretreated with Compound C or not and then exposed to CCL5 as described in methods and materials, the expression of ULK1, CAMKK2, LC3B and phosphorylated AMPK tyrosine 172 were analyzed by western blot. The value of each band indicates the relative expression level after normalizing to the loading control Actin. (k,l) HT29 and DLD-1 cells exposed to CCL5 and then treated with Compound C as described in methods and materials. After that, cell migration was assayed. Each data point was the mean ± SEM of three independent experiments and presented relative to the controls. **P < 0.01, #P > 0.05.

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