Figure 6
From: The guanine nucleotide exchange factor Arhgef7/βPix promotes axon formation upstream of TC10
Active TC10 rescues the loss of axons caused in Arhgef7-deficient neurons. (a) Dissociated hippocampal neurons were transfected at 6 h after plating with expression vectors for an shRNA against Arhgef7 (Arhgef7 RNAi) and GFP-TC10F34L or GFP (green). Transfected neurons were analyzed at 3 d.i.v. by staining with an anti-MAP2 (red, dendrites) and the Tau-1 (blue, axons) antibody. The scale bar is 20 μm. (b) The formation of axons was analyzed at 3 d.i.v. by counting the number neurons without an axon (0, black) with a single axon (1, gray), and with multiple axons (>1, white) (ANOVA, Student’s t-Test, **p < 0.01, n = 3 experiments with 50 neurons counted per condition).
