Figure 3 | Scientific Reports

Figure 3

From: Death-associated protein kinase 1 mediates interleukin-1β production through regulating inlfammasome activation in Bv2 microglial cells and mice

Figure 3

DAPK1 is involved in Aβ25–35-induced IL-1β maturation and caspase-1 activation in LPS-primed Bv2 cells. (A) Confirmation of DAPK1 knockdown and cDAPK1 expression in Bv2 cells by western blotting analysis (see original blots in Supplementary Fig. S3A). (B) The effects of DAPK1 knockdown and cDAPK1 expression on Aβ25–35-induced IL-1β secretion were measured by ELISA. (C–E) The effects of DAPK1 knockdown on LPS-induced TNF-α and IL-6 secretion, as well as LPS + Aβ25–35-induced LDH release were determined. (F–J) The effects of DAPK1 knockdown and cDAPK1 expression on the expression of caspase-1, pro-IL-1β, NLRP3 and ASC in LPS-primed cells were determined by western blotting analysis (see original blots in Supplementary Fig. S3B). Results are mean ± SEM for at least three independent experiments. **P < 0.01, ***P < 0.001; ###P < 0.001. cDAPK1: constructively activated DAPK1 construct; Scr: scrambled shRNA; ShDAPK1: DAPK1-specific shRNA.

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