Figure 3
Full-stage tracking of osteoclast fusion. Co-cultured BMMs from Ctsk-Cre mice and RosamTmG mice were induced with RANKL. GFP+ cells were observed on the third day, most of them containing only one single nucleus (A). Seven days after osteoclastogenic induction, fused multinucleated GFP+ cells were found in proximity to each other (E). From day3 to the final day of observation, GFP+ cells increased in size and became more polarized, with an increased number of nuclei (A–H). From day 7, nuclei (some of them condensed) were observed scattering with the cells. Also, on day 7 and day 8, the number of TG cells possessing both EGFP and tdTomato substantially increased and became easily observable (E–H). Scale bar: 50 um.
