Figure 2

Knockdown of APOC1, CMTM6, FABP4 and WBP5 reduces DiI-LDL uptake into primary human monocyte-derived macrophages. Bars reflect mean ± S.D relative change in cellular DiI-LDL fluorescence signal intensities as determined from cultured primary human monocyte-derived macrophages during the replication screen. Cells were solid-phase transfected with siRNAs against indicated genes (2 independent siRNAs/gene), and DiI-LDL uptake was measured relative to control siRNA treated cells with 1.0 being the mean intensity of all negative control wells for each plate. Shown are results from cells of 10 healthy individuals (grey) and 10 patients with coronary artery disease (orange). To determine significance, deviation values (Dev; see Methods) were calculated for each siRNA tested in each individual relative to the mean intensity of respective negative control wells, and the median deviation was calculated for the control group (Md(C)), the patient group (Md(P)), and the combination of both groups (Md(C + P)) separately. Finally, the mean median deviation shown was calculated from the median deviation of results from three technical replicates per individual (i.e, three independent parallel analyses of cells isolated from the same blood draw). **Dev <−1.0 for at least 2 siRNAs in 1 group (controls, patients, controls + patients). For full siRNA screening results from primary and replication screens, see Supplemental Data.