Figure 2

Identification of CTCs using single-cell RNAseq. Top panels show candidate CTCs identified by Imagestream in patients 1 (A) and 2 (B). (C,D) PCA plots after outlier-detection normalization for each patient, including the loadings for the top marker genes of PC1, and a t-SNE plot of non-CTCs cells with predicted clusters based on the top-ranked marker genes (RBC: red blood cells, NK: Natural Killer, DC: dendritic cells).