Figure 1

N. brasiliensis L3 antigen decreases HeLa migration and EMT marker expression. (A) Scratch motility assay showing HeLa cells exposed to 10 µg N. brasiliensis L3 antigen imaged at 4 and 8 hours post-wound formation and (B). Total area migrated calculated relative to wound area at time 0 hours for HeLa cells exposed to 0.1, 1 and 10 µg N. brasiliensis L3 antigen. (C) Scratch motility assay showing HeLa cells exposed to 10 µg H. polygyrus somatic antigen or 10 µg H. polygyrus excretory/secretory products (HES) imaged at 4 and 8 hours post-wound formation. (D) Transwell migration of HeLa cells determined at 24 hours following exposure to 10 µg N. brasiliensis L3 antigen or an untreated control. (E) Western blot analysis of N-cadherin, vimentin and p38 (loading control) was performed 12 or 24 hours following exposure of HeLa cells to 10 μg or 50 µg N. brasiliensis L3 antigen or an untreated control and (F). Densitometry readings were obtained using ImageJ in order to determine the changes in protein expression of N-cadherin and vimentin normalised to p38 and expressed as a fold-change relative to control wells. (G) Live cell counts over a 3-day period following exposure of HeLa cells to 0.1 µg, 1 µg and 10 µg N. brasiliensis L3 antigen or an untreated control. The figures shown are the result of (G). Two independent experiments, (A–D and F). Three independent experiments or (E). Representative of two independent experiments. The data was analysed using GraphPad Prism 6.01 and a parametric unpaired t-test was performed where ns = not significant, *p < 0.05 **p < 0.01 and ***p < 0.001. Error bars represent Standard Error of the Mean.