Figure 3
Knockdown of miR-301a inhibited cell proliferation, colony formation, cellular migration and promoted doxorubicin-induced cell apoptosis in arsenic transformed BEAS-2B cell. Arsenic transformed BEAS-2B cells were transfected with Anti-control (Anit-control) or LNA-Anti-miR-301a (Anti-miR-301a). (A) Cell proliferation was determined by using cell counting kit-8 at the indicated times. (B) Cells were seeded in soft agar for 4 weeks and the number of colonies in the field were counted. (C) Cell migration was determined by using trans well assay. (D) Migratory cells were counted from (C). (E) Cell apoptosis was measured by using annexinv and PI staining. Doxorubicin (0.2ug/ml) was used to induced cellular apoptosis for 24 h. (F) Apoptotic cells were counted from (E). Values represented the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 indicate a significant difference between Anti-control and Anti-miR-301a transfection groups.
