Figure 3

OE GRG5 ESCs present increased migration capacity and resistance to DNA damage. (a) Wound healing assay indicating increased migration capacity of OE GRG5 ESCs. Graph and phase contrast images presenting the number of migrated cells inside the gap area 24 h upon wound generation. Mean + SD of n = 3 independent experiments. *P < 0.05 (Scale bar, 50 μm) (b) Transwell assay showing the elevated migration capability of OE GRG5 ESCs. Quantification and photos of the DAPI stained migrated nuclei. Mean + SD of n = 3 independent experiments. *P < 0.05 (Scale bar, 100 μm) (c) Analysis of EMT related markers. Western blot for the epithelial marker CDH1 in CONTROL and OE GRG5 ESCs. qPCR analysis of the EMT mediator Zeb1. Mean + SD of n = 3 independent experiments. *P < 0.05 (d) Apoptosis assay demonstrating the resistance of OE GRG5 ESCs to DNA damage. Photos and quantification of the apoptotic CONTROL and OE GRG5 ESCs 22 h upon induction of DNA damage with 0.17 μM Etoposide treatment. *P < 0.05 (Scale bars, 100 μm and 30 μm).