Figure 2 | Scientific Reports

Figure 2

From: Clinical evaluation of rapid fluorescent diagnostic immunochromatographic test for influenza A virus (H1N1)

Figure 2

The limit of detection (LOD) of assays. (A) The performance of rapid diagnostic kit (RDT). After preparing two-fold dilutions of samples ranging from 20 HA/mL to 320 HAU/mL titers, 75 μL of the virus diluent was tested for 20 min. (B) The performance of FICT employing Eu NP. Fluorescent intensities of TL and CL on strips were captured at different H1N1 virus after application of two-fold dilutions of samples ranging from 10 HAU/mL to 160 HAU/mL titers. Data (n = 3) are shown as mean ± SD. (C) Determination of RNA copy number and threshold cycle (Ct) of LOD of FICT. The virus titer corresponding to LOD of FICT was subjected to RNA extraction and used for rRT-PCR. The linear relationship between Ct value and RNA copy number is shown in right panel. Arrow indicates the virus titer of LOD of FICT. NTC, no template control; ①, H1N1; ②, Influenza A; ③, Influenza B; ④, Control line.

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