Figure 3

Analysis of FICT assay with patient specimens. (A) The accuracy of FICT performance was determined with the area under the ROC curve (AUC) by testing negative and positive patients for H1N1 rRT-PCR. (B) Based on the ROC curve analysis, 22.5 was determined as the threshold cut-off value to differentiate H1N1 infection. H3N2-positive patients (n = 10) were additionally tested in FICT. All fluorescent levels of TL/CL were plotted to predict influenza A infection.