Figure 2

The apoptosis of H22 cells induced by PFEE-C and PFEE-W treatment. Different concentrations of PFEE-C and PFEE-W were used to treat H22 cells for 24 h. (a) The apoptosis and necrosis of H22 cells were analyzed by flow cytometry. The individual dot plots were shown in left panels and the summary data were shown in right panels. (b) The nuclear morphology of H22 cells. The above H22 cells were stained with Hoechst 33258 and observed by inverted fluorescence microscopy. The arrows indicated the chromosomal condensation. Data are from 3 independent experiments and analyzed by ANOVA. *p < 0.05; **p < 0.01 compared to untreated group.